Drosophila gut can be found in a recent review by B. Data provided are for informational purposes only. Personal subscribers to Nature can view articles published from to the current issue. Abstract The precise and systematic digestion and subsequent absorption of nutrients in food is vitally important for the life of all metazoans. EGF signaling regulates the proliferation of intestinal stem cells in Drosophila. Relevant information on this subject is emerging from studies of the response to exposure to insecticidal proteins from the bacterium Bacillus thuringiensis Bt as model intoxicants.
The adult Drosophila posterior midgut is maintained by pluripotent stem cells
This article contains supporting information online at www. From a 10x PBS stock solution prepare ml 1x PBS 1. Cookies are used by this site. The dissected midguts must be kept in a cool environment to avoid tissue degradation and reduce RNAse activity in the tissue. It has been noted that copper cells and gastric parietal cells share strikingly similar cell morphology and function, suggesting that these two cell types are analogous 8 , We measured the radius of the gut along its length.
Identification of adult midgut precursors in Drosophila
The only marker that unambiguously identifies ISCs in an old midgut is phospho-histone3 PH3 , since ISCs are the only dividing cells in the midgut 12, FAC sorting strategy to identify and isolate ISCs and EBs from young 7 days midguts. Lin G , Xu N , Xi R Paracrine Wingless signalling controls self-renewal of Drosophila intestinal stem cells. Lee T , Luo L Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Link to citation list in Scopus.
Intestinal stem cells in the adult Drosophila midgut
Description: By using this approach, two different cell lineages are predicted. We therefore tested the relevance of Wnt signaling to maintain GSSCs in the adult Drosophila stomach. If these parameters are not set properly, the sorted cell population will most likely contain dead cells and debris Figure 5B, C , many GFP-positive cells will be missed Figure 5D and the two distinct peaks for GFP-positive cells as seen in the histogram plot Figure 2E and Figure 3E will not be detected. Cells exhibiting lower GFP intensity are small and less granular and represent ISCs. Although trypsin is a very potent enzyme and could have detrimental effects on cells, we still obtained enough living, healthy cells for subsequent FAC sorting.